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间充质干细胞-脂肪分化培养基AdipoLife ™ DfKt ™-2 Adipogenesis Medium Kit

更新日期:2014年03月21日 浏览次数:
名称:间充质干细胞-脂肪分化培养基AdipoLife ™ DfKt ™-2 Adipogenesis Medium Kit

货号:LL-0059

英文名称:AdipoLife DfKt-2 Adipogenesis Medium Complete Kit, Product Code:LL-0059

价格:2292.6RMB

产品说明书:点击下载
产品详情


AdipoLife™ DfKt™-2 Adipogenesis Medium differentiates Human Mesenchymal Stem Cells (HMSC) derived from bone marrow (BM) and Wharton’s Jelly (WJ) into adipocytes.

AdipoLife™ DfKt-2™ is offered in a kit format composed of basal medium (100mL)  and a combined solution of differentiation factors called DifFactor™ 3. This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance. AdipoLife™ Basal Medium comes in a 100 mL bottle.

DifFactor™ 3 contains:

  • rh Insulin
  • Indomethacin
  • 3-Isobutyl-1-Methylxanthine
  • Dexamethasone
  • Horse Serum (5% final conc.)
  • L-Glutamine
  • Ascorbic Acid

AdipoLife™ Basal Medium contains no antimicrobials and no phenol red. Antimicrobials are not required, but are recommended during differentiation due to the duration of culture and the frequency of handling of the cultures. Lifeline®offers antimicrobials and phenol red separately.

AdipoLife™ DfKt-2™ shows efficient differentiation as demonstrated by the accumulation of lipid vesicles.

adipogenesis medium for Bone Marrow Stem Cells

Shown above, left: Wharton’s Jelly Mesenchymal Stem Cells, passage 3, 5 days after inoculation with 5,000 cells/cm2(100X).

Shown above, right: Wharton’s Jelly Mesenchymal Stem Cells differentiated into Adipocytes using AdipoLife™ DfKt™-2 and stained with Lifeline® Oil Red O Lipid Staining Kit, post 4 weeks differentiation (100X).

ISO-Style Testing for Guaranteed Consistency and Reproducible Results

AdipoLife™ DfKt™-2 Adipogenesis Medium  is quality tested for:

  • Sterility:  Negative for bacterial and fungal growth
  • pH: 7.6 +/- 0.3
  • Cell Performance: Adipogenesis of human mesenchymal stem cells
  • Osmolality: 255 +/- 15 mOsm
  • Endotoxin: < 0.5 EU/mL

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