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StemLife PA Pre-Adipocyte Medium Complete Kit

更新日期:2014年03月21日 浏览次数:
名称:StemLife PA Pre-Adipocyte Medium Complete Kit

货号: LL-0058

规格: 500,000 cells

价格: 1591.00RMB

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相关产品:     培养试剂

StemLife PA culture medium is optimized for the undifferentiated expansion of Human Mesenchymal Stem Cells – Pre-Adipocytes under low serum (2%) conditions. StemLife PA supports the undifferentiated growth of these cells at rates equal to or greater than comparable media supplemented with up to 10% FBS.

StemLife PA medium, when used with Lifeline® Human Pre-Adipocytes, provides an ideal culture model for the study of diabetes, obesity, metabolism, insulin sensitivity, and adipose biology. Lifeline® Human Pre-Adipocytes can be differentiated down chondrogenic, and osteogenic lineages, and therefore provide a model for the study of cell differentiation.

StemLife PA Basal Medium contains no antimicrobials and no phenol red.  These components are available from Lifeline®, but are not required for eukaryotic cell proliferation.

  • Kit format for formulation flexibility: Offered in a kit format composed of basal medium and associated supplements and growth factors called “LifeFactors®.” This kit allows you to prepare fresh medium in your laboratory, extending shelf life and enhancing performance.
  • Protective packaging: StemLife PA Basal Medium is provided in a light-protected 500 mL bottle. The remaining LifeFactors®are packaged in a convenient gas-impermeable pouch for easy storage.

Pre-Adipocyte Culture Medium

 

pre-adipocyte cultureStemLife PA cell culture medium grows Human Mesenchymal Stem Cells – Pre-Adipocytes in low-serum conditions while maintaining excellent cell morphology, and maintaining an undifferentiated state.  Human Pre-Adipocytes can then be differentiated as seen below.

Shown at left: Human Pre-Adipocytes, passage 4, 4 days after inoculation with ~5,000 cells/cm2 (100X)

Shown below (from left to right): Lifeline® Human Pre-Adipocytes differentiated to adipocytes (stained with Oil Red O, 100X), osteoblasts (stained with Alizarin Red S, 100X), and chondrocytes (sliced and stained with Alcian blue, 400X).


pre-adipocyte differentiation

Every production lot of StemLife PA culture medium is extensively tested using Human Mesenchymal Stem Cells – Pre-Adopocytes. StemLife PA Medium and Gentamicin-Amphotericin B are cell culture tested separately.

  • Sterility Testing:  Negative for bacterial and fungal growth
  • pH: 7.4 +/- 0.2
  • Cell Testing: Rate of proliferation and morphology
  • Osmolality: 270 +/- 10 mOsm
  • Endotoxin: < 0.5 EU/mL

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